Regulatory

Part:BBa_K4140011

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-24)
Revision as of 18:58, 11 October 2022 by Mohamed-Ab0elghare (Talk | contribs) (→‎Usage)

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Lac promoter

Part Description

A CAP binding site and the lac operator sequence lacO are present in this lac promoter. Although the lac promoter is not as potent as the tac or trc promoters, it nevertheless provides for decent quantities of foreign protein production in high copy-number vectors. Expression from the lac promoter is activated in the absence of the lac repressor (LacI).

Usage

We use the lac promoter as a constitutive promoter for the regulation of (Phenylalanine-specific permease)expression even if it is not as powerful as the tac or trc promoters. It still shows significant levels of phenylalanine enough to induce and start our circuit activity by improving the permeability of the membrane to phenylalanine as shown in figure 1.

Figure(1) Shows an SBOL demonstrating the usage of permease with lac promoter in our whole cel-based biosensor











Experimental Characterization

Tube131.png








This figure shows an experimental characterization of this part as it's validated through gel electrophoresis as it is in lane 7. The run part (ordered from IDT) included Lac promoter - Permease.













References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None