Composite

Part:BBa_K4139001:Design

Designed by: Emily Hagens   Group: iGEM22_Lethbridge   (2022-10-11)
Revision as of 18:49, 11 October 2022 by Emily Hagens (Talk | contribs) (→‎References)

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mcyH ABC Transporter for Microcystin Production (Composite)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 789
    Illegal AgeI site found at 113
    Illegal AgeI site found at 1177
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon-optimized for use in E.coli, BioBrick assembly standard


Source

GenBank: AAF00956.1

References

Pearson LA, Hisbergues M, Börner T, Dittmann E, Neilan BA. Inactivation of an ABC transporter gene, mcyH, results in loss of microcystin production in the cyanobacterium Microcystis aeruginosa PCC 7806. Appl Environ Microbiol. 2004 Nov;70(11):6370-8. doi: 10.1128/AEM.70.11.6370-6378.2004. PMID: 15528494; PMCID: PMC525210.