Device

Part:BBa_K283023

Designed by: Liu Chenli   Group: iGEM09_HKU-HKBU   (2009-10-11)
Revision as of 03:10, 12 October 2009 by Registry (Talk | contribs)

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plux-rbs-CI-terminator-plambda(R-O12)-rbs-cheZ-terminator

This part contains a strong rbs and a TetR tagged with a LVA degradation tail. The TetR repressor can be used to repress ptet promoter and the TetR coding region do not contain any terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal NheI site found at 2472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal BglII site found at 332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/device/uncategorized
//function/motility
Parameters
None