Part:BBa_K4274000

ClSS_S533A

ClSS_S533A

Usage and Biology

ClSSS533A is an optimized biobrick part encoding the gene for alpha-santalene synthase from Clausena lansium. The enzyme catalyzes the conversion of the common isoprenoid intermediate farnesyl pyrophosphate (FPP) into the alpha-santelene in a single step. It is reported that ClSS's basic amino acid residue S533’s mutation to typical nonpolar amino acid Ala could result in a 1.7-fold increase in production of alpha-santalene compared to the nonmutated strain (Jia Z.et al., 2022) .
This year, we are using ClSSS533A to construct composite parts ptac-RiboJ-B0034-ClSSS533A-B0034-ERG20-B0015 (part: BBa_K4274020), ptac-RiboJ-B0034-ClSSS533A-B0034-ERG20F96W-B0015 (part: BBa_K4274021) and ptac-RiboJ-B0034-ClSSS533A-FL-ERG20F96W-B0015 (part: BBa_K4274023). This will allow engineering E.coli DH5a (tnaA-) to heterologously express ClSSS533A. Other teams can utilize this part for E. coli alpha-santalene production.

Source

Clausena lansium

Characterization

Test

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 183
  
• 1000
  COMPATIBLE WITH RFC[1000]