Coding

Part:BBa_K4117888:Design

Designed by: He Du,Yue Zhang   Group: iGEM22_BNU-China   (2022-09-30)
Revision as of 17:47, 11 October 2022 by Qiyueyao (Talk | contribs) (→‎Source)


UGT1A3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4599
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4599
    Illegal BglII site found at 4257
    Illegal BamHI site found at 2887
    Illegal BamHI site found at 3444
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
    Illegal AgeI site found at 3031
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 732


Design Notes

To degrade Δ9-THC, we added a pathway to sense Δ9-THC in front of the UGT1A3 gene.

Source

We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P35503

C-phycocyanin alpha subunit and beta subunit genes come from Part:BBa_K4117779.

PmrC promoter comes from Part:BBa_K1459006.

PmrA gene comes from Part:BBa_K3611003.

OmpT signal peptide gene comes from Part:BBa_K3805532.

OmpA signal peptide gene comes from Part:BBa_K103006.

T7 terminator gene comes from Part:BBa_B0012.

rrnB T1 Terminator gene comes from BBa_K3257020.

References