Coding

Part:BBa_K4117088:Experience

Designed by: Yueyao Qi,Bojun Xiong   Group: iGEM22_BNU-China   (2022-09-30)
Revision as of 17:37, 11 October 2022 by Qiyueyao (Talk | contribs) (→‎Applications of BBa_K4117088)


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Applications of BBa_K4117088

“T7 promoter+ PmrA+ RBS+ PmrB+ rnaB T1 terminator+ pmrC promoter” is the THC sensing pathway. To alert vaccinators that they have ingested THC, we design a THC sensing system. In this pathway, anti-THC can specifically bind to THC, and then promote PmrB phosphorylation of PmrA. PmrC promoters activate phosphorylated PmrA proteins and initiate transcription to express pigment proteins. Pigment proteins can be excreted in the stool, acting as a warning.

Outer membrane protease T (OmpT) is a 33.5 kDa endoprotease located on the outer membrane of Escherichia coli. OmpT signal peptide is an excretion tag linked on the N-terminus of protein. It can transport proteins across the outer membrane with the help of OmpT.

Δ9-THC is hydroxylated to 11-OH-Δ9-THC via CYP2C9 and CYP2C19, remaining psychoactive.

User Reviews

UNIQ3c482fee9db5d525-partinfo-00000000-QINU UNIQ3c482fee9db5d525-partinfo-00000001-QINU