Part:BBa_K4414003

NES

This nuclear export signal（NES）directs the protein out of the nucleus.

Figure 1.This is the sequence of NES

Usage and Biology

Regulating the output of many proteins from the nucleus depends on the presence of a nuclear export signal(NES) consisting of leucine-rich amino acids.(Fornerod et al., 1997;Fischer et al., 1995). The shuttle receptor that appears to bind to the NES sequence and function in the protein output from the nucleus to the cytoplasm is CRM1 (chromosomal region maintenance)/export protein .(Richards et al., 1997) CRM1 binds to Ran GTPase and interacts with the nucleoporous components to translocate NES-containing proteins to the cytoplasm(Stade et al., 1997).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

1.Fischer, U., Huber, J., Boelens, W. C., Mattaj, I. W., & Lührmann, R. (1995). The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell, 82(3), 475–483. https://doi.org/10.1016/0092-8674(95)90436-0

2.Fornerod, M., Ohno, M., Yoshida, M., & Mattaj, I. W. (1997). CRM1 is an export receptor for leucine-rich nuclear export signals. Cell, 90(6), 1051–1060. https://doi.org/10.1016/s0092-8674(00)80371-2

3.Richards, S. A., Carey, K. L., & Macara, I. G. (1997). Requirement of guanosine triphosphate-bound ran for signal-mediated nuclear protein export. Science (New York, N.Y.), 276(5320), 1842–1844. https://doi.org/10.1126/science.276.5320.1842