Generator

Part:BBa_K201000

Designed by: francesca ceroni   Group: iGEM09_Bologna   (2009-10-11)
Revision as of 21:58, 11 October 2009 by Registry (Talk | contribs)

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LacI repressed GFP generator with BBa_K201007

GFP reporter protein was cloned under the control of the BBa_J23118 costitutive promoter from the Berkley library (1429). The promoter is regulated by Lac repressor natural operator O2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


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Categories
Parameters
n/aLacI repressed GFP generator with BBa_K201007