Coding

Part:BBa_K4117088:Design

Designed by: Yueyao Qi,Bojun Xiong   Group: iGEM22_BNU-China   (2022-09-30)
Revision as of 17:26, 11 October 2022 by Qiyueyao (Talk | contribs) (→‎Design Notes)


CYP2C19


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4467
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
    Illegal PstI site found at 3118
    Illegal PstI site found at 4348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4467
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
    Illegal PstI site found at 3118
    Illegal PstI site found at 4348
    Illegal NotI site found at 3275
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4467
    Illegal BglII site found at 4386
    Illegal BamHI site found at 2887
    Illegal BamHI site found at 2994
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4467
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
    Illegal PstI site found at 3118
    Illegal PstI site found at 4348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4467
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
    Illegal PstI site found at 3118
    Illegal PstI site found at 4348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 732


Design Notes

To degrade Δ9-THC, we added a pathway to sense Δ9-THC in front of the CYP2C19 gene.

Source

how to make CYP2C19

References