Coding

Part:BBa_K4414008

Designed by: Yue Yin   Group: iGEM22_NUDT_CHINA   (2022-09-22)
Revision as of 17:24, 11 October 2022 by TheaYang (Talk | contribs)


P2A

This composite part consists of a TCE promoter(Part:BBa_K4016011) and a coding sequence of tdTomato(Part:BBa_K4414007).This is a reporter gene that is used to make a visual response to stimulation of upstream genes.

Usage and Biology

Two genes(ORF) becomes an ORF through the 2A polypeptide chain ligation, and the mRNA is translated into fusion protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None