Part:BBa_K4169006

BBa_K4169006

HepT: a RNase like toxin

HepT is a toxin that can be effectively inhibited the growth of bacteria.The HepT toxin could function as an RNase with a RX4-6H active motif and cleave mRNA to inhibit cell growth. Complete with HepT (HZAU-China 2021:BBa_K3733010) was used in this study.

Usage and Biology

As an RNase enzyme, HepT can degrade mRNA at the transcriptional level, which ultimately leads to cell death

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 103
  Illegal PstI site found at 127
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 103
  Illegal PstI site found at 127
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 103
  Illegal PstI site found at 127
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 103
  Illegal PstI site found at 127
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Yao J, Zhen X, Tang K, et al. Novel polyadenylylation-dependent neutralization mechanism of the HEPN/MNT toxin/antitoxin system[J]. Nucleic acids research, 2020, 48(19): 11054-11067. [2] Yao J, Guo Y, Zeng Z, et al. Identification and characterization of a HEPN‐MNT family type II toxin–antitoxin in S hewanella oneidensis[J]. Microbial biotechnology, 2015, 8(6): 961-973. [3] Jia X, Yao J, Gao Z, et al. Structure–function analyses reveal the molecular architecture and neutralization mechanism of a bacterial HEPN–MNT toxin–antitoxin system[J]. Journal of Biological Chemistry, 2018, 293(18): 6812-6823.