Part:BBa_K4169005
RNA Thermometer
Under natural conditions, some RNA with special structures can form stem loops autonomously. Complete with RNA Thermometer(HZAU-China 2021:BBa_K3733011) was used in this study.
Usage and Biology
The RNA thermometer has a special sequence capable of forming stem-loop structures at 27 ° C and below and opening at 37 ° C. At the same time, there is a specific binding site for RNase E on the sequence that can form the secondary structure. At the transcriptional level, if this sequence is present on mRNA, it is able to bind RNase E and thus degrade the entire RNA strand. Therefore, we introduce the thermometer into the suicide module to achieve temperature sensitive suicide.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 39
References
Hoynes-O'Connor A, Hinman K, Kirchner L, Moon TS. De novo design of heat-repressible RNA thermosensors in E. coli.[J] Nucleic Acids Research. 2015 Jul 13;43(12):6166-79.
| biology | Escherichia coli |