Coding

Part:BBa_K4256122

Designed by: Xing Zhu   Group: iGEM22_UCAS-China   (2022-09-28)
Revision as of 16:46, 11 October 2022 by SunJi UCAS (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

ccdB

It is a component of the CcdA/CcdB Type II Toxin-antitoxin (TA) system. It encodes the CcdB poison, which is a potent inhibitor of cell proliferation. In absence of the antitoxin, the CcdB poison traps DNA-gyrase cleavable complexes, inducing breaks into DNA and cell death. As a model system, by ensuring an antidote-toxin ratio greater than one, this mechanism might prevent the harmful effect of CcdB in plasmid-containing bacteria. The CcdA-CcdB toxin-antitoxin system we used is a killing switch that is relatively nontoxic to human and other mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 40
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None