Composite

Part:BBa_K4228017:Hard Information

Designed by: Keyao Zhao   Group: iGEM22_Tongji_China   (2022-09-30)
Revision as of 16:10, 11 October 2022 by Fork (Talk | contribs) (→‎Brife introduction)

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/*Brife introduction*/ pET-32a is a T7 promoter vector which can propagate in Escherichia coli after appropriate induction by IPTG.The desired polypeptide can be expressed as a fusion protein with 6xHis tag at the C-terminus for simplified purification. In order to complete the heterogenous expression of mutant bromelain in Bacillus subtilis, we cloned the sequence as BamHI-XhoI inserts in the pBER-2 expression vector as well. pBER-2 is a P43 promoter based shuttle vector which can propagate in Escherichia coli and Bacillus subtilis.The desired polypeptide can be expressed as a fusion protein with His6 tag at the C-terminus and Alkaliophilic Bacillus alkaline protease signal peptide and propeptide at the N-terminus to induce protein secretion.