DNA

Part:BBa_K4162118

Designed by: Weiwen Chen   Group: iGEM22_Fudan   (2022-10-09)
Revision as of 15:57, 11 October 2022 by Ted Chen (Talk | contribs)


ribozyme+RBS+CDS module: crtEBIY

Introduction

2021 Fudan

This biobrick was created through overlapping PCR of BBa_K4162009(ribozyme+B0_RBS+crtE), BBa_K4162013(ribozyme+T7_RBS+crtB), BBa_K4162016(ribozyme+T7_RBS+crtI) and BBa_K4162019(ribozyme+T7_RBS+crtY). These genes are a part of the carotenoid biosynthesis pathway and together, this biobrick converts farnesyl pyrophosphate to beta-carotene. In this part, the RNA sequences of hammerhead ribozyme conduct self-cleaving, and the polycistronic mRNA transcript is thus co-transcriptionally converted into individual mono-cistrons in vivo. Self-interaction of the polycistron can be nullified and each cistron can initiate translation with comparable efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2202
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1681
    Illegal NgoMIV site found at 1811
    Illegal AgeI site found at 839
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None