Coding

Part:BBa_K4165015

Designed by: Hossam Hatem   Group: iGEM22_CU_Egypt   (2022-09-29)
Revision as of 15:51, 11 October 2022 by SalmaMohsen 2000 (Talk | contribs)


UBE2W

Ubiquitin-conjugating E2 ligase that has a role in the ubiquitination cascade for protein degradation.

Usage and Biology

This E2 ubiquitin-conjugating enzyme UBE2W is the key participant in the trio of enzymes as it is responsible for the monoubiquitylation of TRIM21 E3 ligase to initiate its polyubiquitylation by the UBE2N/UBE2V2 heterodimer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

WetLab Results

Transformation of His UBE 2w in BL-21 using pGS-21a vector 

                             Figure 2. Transformed plate of His UBE 2w + pGS-21a

Transformation of His UBE 2w in DH-5 alpha using pJET vector

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                             Figure 3. Transformed plate of His UBE 2w + pJET

References

1. Stewart, M. D., Ritterhoff, T., Klevit, R. E., & Brzovic, P. S. (2016). E2 enzymes: more than just middle men. Cell research, 26(4), 423-440.

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