Coding

Part:BBa_K4239007:Design

Designed by: Guillaume FULCONIS   Group: iGEM22_INSA_Lyon1   (2022-10-08)
Revision as of 15:47, 11 October 2022 by Gfulconis (Talk | contribs) (→‎References)


Enhanced units fiatluxABE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2853
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 504
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1023


Design Notes

Mutations realised in the composite part fiatluxABE are explained on the page of the basic parts fiatluxA (BBa_K4239003), fiatluxB (BBa_K4239004) and fiatluxE (BBa_K4239005).

Source

azr

References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.