Translational_Unit

Part:BBa_K4239006:Design

Designed by: Guillaume FULCONIS   Group: iGEM22_INSA_Lyon1   (2022-10-08)
Revision as of 15:40, 11 October 2022 by Gfulconis (Talk | contribs) (→‎References)

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Enhanced luciferase substrate forming subunits fiatluxCD and the promoter J23117


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 50
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1366


Design Notes

Mutations realised in the composite part fiatluxCD are explained on the page of the basic parts fiatluxC (BBa_K4239001) and fiatluxD (BBa_K4239002).

Source

The source of fiatluxCD is synthesis. This part was synthetized from the nucleotidique sequence according to Gregor et al.’s study in 2018.

This part was synthetized directly with the promoter BBa_J23117.

References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.