Coding

Part:BBa_K4165254

Designed by: Esraa Elmligy   Group: iGEM22_CU_Egypt   (2022-10-08)
Revision as of 15:38, 11 October 2022 by SalmaMohsen 2000 (Talk | contribs)


GST-Coh2

This part encodes Cohesin 2 protein tagged with GST for its purification and characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85

WetLab Results

Transfotmation of GST COH in BL-21 using pGS-21a vector 

                                          Figure 2. Transformed plate of GST COH + pGS-21a

Transformation of GST COH in DH-5 alpha using pJET vector 

                                        Figure 3. Transformed plate of GST COH + pJET

Comparison between chemical lysis and sonication for GST COH

Figure 4. This graph shows a highly significant difference between the chemical lysis and sonication for GST COH, after we had this result we optimized our protocol to use sonication for GST COH

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Categories
Parameters
None