Coding
Part:BBa_K4365004
Designed by: Giorgio Gilioli Group: iGEM22_TU_Dresden (2022-09-30)
Revision as of 15:37, 11 October 2022 by Giorgio TUD 2020 (Talk | contribs)
SMT3 (SUMO)
SMT3 protein is a Ubiquitin-like protein of yeast origin. The SUMO gene commonly used is a gene of yeast origin called Smt3. When a protein fused to this tag is expressed in a prokaryote like E. coli, the cells need to be lysed and then the protein is purified and treated with the SUMO protease Ulp1, which is also of yeast origin.
The cleavage site recognized by SUMO protease is evolutionarily conserved and is located at the last two glycine residues of the SUMO tag. This is where the SUMO protease carries out a proteolytic reaction that dissociates the tag from the downstream cargo protein. It can aminoterminally fused to a protein and can be removed from it without leaving aminoacids [1].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
- ↑ Hickey CM, Wilson NR, Hochstrasser M. (2012) Function and regulation of SUMO proteases, Nature reviews. Molecular Cell Biology.;13(12):755-766. DOI: 10.1038/nrm3478. PMID: 23175280; PMCID: PMC3668692.
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Categories
Parameters
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