Coding

Part:BBa_K4414008

Designed by: Yue Yin   Group: iGEM22_NUDT_CHINA   (2022-09-22)
Revision as of 15:35, 11 October 2022 by TheaYang (Talk | contribs)


P2A

This composite part consists of a TCE promoter(Part:BBa_K4016011) and a coding sequence of tdTomato(Part:BBa_K4414007).This is a reporter gene that is used to make a visual response to stimulation of upstream genes.

Usage and Biology

Two genes(ORF) becomes an ORF through the 2A polypeptide chain ligation, and the mRNA is translated into 1 fusion protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None