Translational_Unit

Part:BBa_K4239006:Design

Designed by: Guillaume FULCONIS   Group: iGEM22_INSA_Lyon1   (2022-10-08)
Revision as of 15:35, 11 October 2022 by Gfulconis (Talk | contribs) (→‎Design Notes)


Enhanced luciferase substrate forming subunits fiatluxCD and the promoter J23117


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 50
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1366


Design Notes

Mutations realised in the composite part fiatluxCD are explained on the page of the basic parts fiatluxC <a href="https://parts.igem.org/Part:BBa_K4239001" class="pr-0" target="_blank">(BBa_K4239001)</a> and fiatluxD <a href="https://parts.igem.org/Part:BBa_K4239002" class="pr-0" target="_blank">(BBa_K4239002)</a>.

Source

azer

===References===