Coding

Part:BBa_K4165255

Designed by: Esraa Elmligy   Group: iGEM22_CU_Egypt   (2022-10-08)
Revision as of 15:28, 11 October 2022 by SalmaMohsen 2000 (Talk | contribs)


GST-Coh2-G4S- TD28REV

Long description

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 689
    Illegal SapI.rc site found at 85

WetLab Results

Transformation of GST COH (L) TD28 Rev in BL-21 using pGS-21a vector 

                                Figure 3. Transformed plate of GST COH (L) TD28 Rev + pGS-21a

Transformation of GST COH (L) TD21 Rev in DH-5 alpha using pJET vector 

                               Figure 4. Transformed plate of GST COH (L) TD28 Rev + pJET

Comparison between chemical lysis and sonication for GST COH TD28 Rev

Figure 5. this graph shows a difference between chemical lysis and sonication for GST COH TD28 Rev, after we had the results we optimized our protocol to use chemical lysis for GST COH (L) TD28 Rev

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Categories
Parameters
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