Coding

Part:BBa_K258102:Design

Designed by: Cihan Tastan   Group: iGEM09_METU-Gene   (2009-10-10)
Revision as of 22:42, 10 October 2009 by Metugen (Talk | contribs) (→‎References)

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PrtF domain of ABC transporter of Erwinia chrysanthemi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 967
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1329
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene actually will be fully function with other two genes which are PrtD and PrtE.


Source

E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.

References

[1]Protease secretion by Erwinia chrysanthemi: the specific secretion functions are analogous to those of Escherichia coli c!-haemolysin Sylvie Letoffe, Philippe Delepelaire and Cecile Wandersman Unite de Gdnetique Moleculaire (URA CNRS 1149), Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France The EMBO Journal vol.9 no.5 pp. 1 375 - 1382, 1990

[2]http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=protein&dopt=GenPept&RID=D1AV4GK6012&log%24=prottop&blast_rank=3&list_uids=60101758