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Reporter
Part:BBa_K4365003
Designed by: Giorgio Gilioli Group: iGEM22_TU_Dresden (2022-09-30)
Revision as of 15:21, 11 October 2022 by Giorgio TUD 2020 (Talk | contribs)
turboRFP Selection Device with constitutive promoter
Improvement of BBa_J04450 part. In the original construct, mRFP1 expression was observed irrespective of IPTG induction. So we replaced the inducible LacI-regulated promoter (BBa_R0010) with a constitutive (BBa_J23100) for colony screening.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 683
Usage and Biology
[edit]
Categories
Parameters
None |