Part:BBa_K4146101

pLlacO1

"Full wiffelball" with tags of the BMCs under regulation of lambdapLhybrid promotor and LacI promoto. The lactose operon is a group of genes involved in lactose breakdown, consisting of repressors and manipulating sequences of the lactose system, making a set of genes related to lactose metabolism regulated synchronously. It has negative regulation mechanism: induce the inactivation of lactose: repressor protein, shedding from LacO, (+) transcription; lactose deficiency: repressor protein binds to LacO, RNA polymerase cannot bind to LacP, (-) transcription. Positive regulation mechanism: can induce activator: CAPorCRP (cAMP binding protein), CRP-cAMP and initiator gene binding, (+) transcription.

This gene can be found in E. coli Nissle 1917

This gene is present in part<a href = "https://parts.igem.org/Part:BBa_K4146106"> BBa_K4146106</a>,

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]