Coding
Part:BBa_K258101:Design
Designed by: Cihan Tastan Group: iGEM09_METU-Gene (2009-10-10)
PrtE domain of ABC transporter of Erwinia chrysanthemi
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1136
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1136
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1136
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1136
Illegal AgeI site found at 1270 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene actually will be fully function with other two genes which are PrtD and PrtF.
Source
E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.On the basis of the apparent molecular weights we tentatively assigned the 50 kd protein to the prtD gene product (protein PrtD).