Coding

Part:BBa_K4146101

Designed by: Yazhao Liu   Group: iGEM22_BUCT   (2022-09-16)
Revision as of 15:15, 11 October 2022 by LYZ-BUCT (Talk | contribs)


pLlacO1

"Full wiffelball" with tags of the BMCs under regulation of lambdapLhybrid promotor and LacI promoto. The lactose operon is a group of genes involved in lactose breakdown, consisting of repressors and manipulating sequences of the lactose system, making a set of genes related to lactose metabolism regulated synchronously. It has negative regulation mechanism: induce the inactivation of lactose: repressor protein, shedding from LacO, (+) transcription; lactose deficiency: repressor protein binds to LacO, RNA polymerase cannot bind to LacP, (-) transcription. Positive regulation mechanism: can induce activator: CAPorCRP (cAMP binding protein), CRP-cAMP and initiator gene binding, (+) transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None