Coding

Part:BBa_K4309000

Designed by: Yanqiu Tian   Group: iGEM22_NPU-CHINA   (2022-09-29)
Revision as of 15:02, 11 October 2022 by Apillow (Talk | contribs)


Laccase（Bacillus）

Lignin peroxidase (LiP), phenoloxidases (laccases, tyrosinases), manganese dependent peroxidase (MnP) are the three enzymes commonly employed ligninolytic enzymes which are mainly involved in degrading lignin and analogue PAHs. Various researches have revealed that the mechanism of oxidation of PAHs by fungi ligninolytic enzymes is similar to the degradation of nonphenolic lignin.In vitro,laccases has activity towards 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 2,6-dimethoxy-phenol, and guaiacol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1207
    Illegal AgeI site found at 1348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1275
    Illegal BsaI.rc site found at 1509
    Illegal SapI.rc site found at 286


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Parameters
None