Regulatory

Part:BBa_K4365002

Designed by: Giorgio Gilioli   Group: iGEM22_TU_Dresden   (2022-09-29)
Revision as of 14:53, 11 October 2022 by Giorgio TUD 2020 (Talk | contribs)

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2 micron Ori

2 micron Ori The 2 micron ori of Saccharomyces cerevisiae is a multi-copy DNA element that resides in the yeast nucleus at a copy number of 40-60 per haploid cell [1]. The plasmid is able to persist in host populations with almost chromosome-like stability.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 360
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 360
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 360
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. Chan KM, Liu YT, Ma CH, Jayaram M, Sau S. The 2 micron plasmid of Saccharomyces cerevisiae: a miniaturized selfish genome with optimized functional competence. Plasmid. 2013;70(1):2-17. doi:10.1016/j.plasmid.2013.03.001
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