Coding

Part:BBa_K4117002:Design

Designed by: Xiaoshan Luo,Meilin Zheng   Group: iGEM22_BNU-China   (2022-09-30)
Revision as of 14:44, 11 October 2022 by Qiyueyao (Talk | contribs) (→‎Source)


CYP2C9


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
    Illegal NotI site found at 284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1386
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since this subunit has been proved to be expressed successfully, we did not re-engineer this protein gene.

Source

We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from NCBI:P33261.4

References