Tag

Part:BBa_K258006:Design

Designed by: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology   Group: iGEM09_METU-Gene   (2009-10-09)
Revision as of 20:33, 9 October 2009 by Metugen (Talk | contribs) (→‎References)

Thermostable lipase (TliA) of Pseudomonas fluorescens SIK W1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 651
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was optimized for E.coli with Biobrick restriction sites


Source

The thermostable lipase (TliA) of P. fluorescens SIK W1 is comprised of 476 amino acids and has the characteristic C-terminal signal sequence recognized by the ABC transporter.

References


[1]Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD) Chan Woo Chung1, Jinsun You1, Kyeongyeon Kim1, Yuseok Moon2, Hoeon Kim3 and Jung Hoon AhnPublished: 29 January 2009 Microbial Cell Factories 2009, 8:11 doi:10.1186/1475-2859-8-11 Received: 18 November 2008 Accepted: 29 January 2009 This article is available from: http://www.microbialcellfactories.com/content/8/1/11


[2]Ahn JH, Pan JG, Rhee JS: Identification of the tliDEF ABC transporter specific for lipase in Pseudomonas fluorescens SIK W1. J Bacteriol 1999, 181(6):1847-1852.


[3]Guzzo J, Duong F, Wandersman C, Murgier M, Lazdunski A: The secretion genes of Pseudomonas aeruginosa alkaline protease are functionally related to those of Erwinia chrysanthemi proteases and Escherichia coli alpha-haemolysin. Mol Microbiol 1991, 5(2):447-453.