Part:BBa_K4216045

pCOLADuet_Bettencourt

The pDuet_Bettencourt Vectors is a sets of 3 compatible vector for transformation in E.Coli. We re-engineered the pETDuet System (https://www.merckmillipore.com/FR/fr/product/pETDuet-1-DNA-Novagen,EMD_BIO-71146#documentation) and cloned the Transcriptional unit of a GFP from the V35 vector of the CIDAR MoClo[1,2] flanked with BsaI restriction site and barcode A and E of the Moclo for assembly of entire transcriptional unit. Like so, during a Golden Gate Cloning, after the BsaI cut, the GFP is replaced by the insert. This allow for easy selection against Green fluorescence (compatible with the mScarlet reporter used in all of our toolkit)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 1888
  Illegal SpeI site found at 2120
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NheI site found at 1238
  Illegal NheI site found at 2145
  Illegal NheI site found at 2168
  Illegal SpeI site found at 2120
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 1888
  Illegal SpeI site found at 2120
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 1888
  Illegal SpeI site found at 2120
  Illegal AgeI site found at 150
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 223
  Illegal BsaI site found at 3136
  Illegal BsaI.rc site found at 2101