Composite

Part:BBa_K4376003

Designed by: Yunshu Wang   Group: iGEM22_NFLS_Nanjing   (2022-09-07)
Revision as of 13:35, 11 October 2022 by Oliviawwwww (Talk | contribs)


the composite part is used to express the bpsA gene.

Construction of the bpsA gene and tac inducible promoter to control the production of the blue-pigment indigoidine synthetase. The composite part is inserted into C.glutamicum in plasmid, resulting in the successful biosynthesis of indigodine pigment.

We amplified two bpsA fragments then transformed recombinant vector into DH5α E.coli strain and Corynebacterium glutamicum.

Engineering-1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3893
    Illegal XhoI site found at 3046
    Illegal XhoI site found at 3745
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 292
    Illegal NgoMIV site found at 699
    Illegal AgeI site found at 1957
    Illegal AgeI site found at 2010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 235
    Illegal BsaI site found at 1546
    Illegal BsaI site found at 2260
    Illegal BsaI.rc site found at 364
    Illegal BsaI.rc site found at 3550
    Illegal SapI.rc site found at 1798


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