Part:BBa_K4160001

EpoR (erythropoietin receptor)

The erythropoietin receptor (EpoR) is a type I cytokine receptor. This receptor induces downstream signaling after binding of its ligand erythropoietin (Epo).¹ The EpoR forms the foundation of the Generalized Extraceullar Molecule Sensor (GEMS) receptor that was developed by Schneller et al., 2018.² This part encodes for an EpoR construct that has been designed to develop the GEMS receptor. This construct is a truncated form of the EpoR that has been mutated to render it inert to Epo.²

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 289
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 658
  Illegal PstI site found at 289
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 172
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 289
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 289
  
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

The erythropoietin receptor (EpoR) is a type I cytokine receptor,¹ that originates from Homo sapiens (Human).³ The EpoR is predominantly expressed on the surface of immature erythroid cells.¹ Together with its ligand Epo, it is the primary regulator of mammalian erythropoiesis (the production of red blood cells).⁴ Binding of Epo to the EpoR induces proliferation, survival, and differentiation of erythroid progenitors into red blood cells.^1,4
When EpoR is in its inactive form, the EpoR dimers are locked by transmembrane helix interactions. This conformation prevents downstream signaling.² Binding of the hormone Epo induces reorientation and dimerization of two EpoR monomers, which forms an active dimeric receptor structure (Figure 1). Consequently, signal transduction via Janus Kinase 2 (JAK2) is initiated by the intracellular domains of the EpoR.^1,4