Protein_Domain
Fok_a
Part:BBa_K243000:Design
Designed by: Freiburg Bioware09 Group: iGEM09_Freiburg_bioware (2009-10-08)
Protein domain (active) of the restriction endonuclease FokI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 487
Design Notes
Modifications of the vector (catalytic active heterodimer) -heterodimeric aminio acids
* switch Glutamate 490/310-312 to Lysin (GAA->AAA)
* switch isoleucin 538/454-456 to Lysin (ATC->AAA)
Source
extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene
References
Mary C. Looneya, Laurie S. Morana, William E. Jacka, George R. Feeherya, Jack S. Bennera, Barton E. Slatkoa and Geoffrey G. Wilson;(1989) Nucleotide sequence of the FokI restriction-modification system: separate strand-specificity domains in the methyltransferase Gene Vol.80 Issue:2 Pages:193-208