Protein_Domain
Fok_a
Part:BBa_K243000:Design
Designed by: Freiburg Bioware09 Group: iGEM09_Freiburg_bioware (2009-10-08)
Protein domain (active) of the restriction endonuclease FokI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 487
Design Notes
Modifications of the vector (catalytic active heterodimer) -heterodimeric aminio acids
* switch Glutamate 490/310-312 to Lysin (GAA->AAA)
* switch isoleucin 538/454-456 to Lysin (ATC->AAA)
Source
extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene
References
NUCLEOTIDE-SEQUENCE OF THE FOKI RESTRICTION-MODIFICATION SYSTEM - SEPARATE STRAND-SPECIFICITY DOMAINS IN THE METHYLTRANSFERASE Author(s): LOONEY, MC; MORAN, LS; JACK, WE, et al.GENE Vol.80 Issue:2 Pages:193-208 Published:AUG 15 1989