Protein_Domain

Part:BBa_K4181026

Designed by: Junzhi Yan   Group: iGEM22_SJTU-BioX-Shanghai   (2022-10-11)
Revision as of 11:54, 11 October 2022 by Registry (Talk | contribs)

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Dsup- K198M_V195R

We directed the evolution of the DSUP protein to convert lysine at position 198 to methionine and valine at position 195 to arginine.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 515
    Illegal PstI site found at 1139
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 515
    Illegal PstI site found at 1139
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 163
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 515
    Illegal PstI site found at 1139
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 515
    Illegal PstI site found at 1139
    Illegal AgeI site found at 121
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 197


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Categories
Parameters
None