Part:BBa_K4146507
pCS-aroGfbr
BUCT hopes to use 2-PE as an example to give E. coli Nissle 1917 the ability to sweeten hair in addition to improving scalp microecology. We use the natural phenylalanine synthesis pathway of E. coli to synthesize 2-PE. aroG was used to enhance the accumulation of a key intermediate, DAHP. aroG, as a metabolic pathway gene that has attracted much attention for many years, has always been a worthy object of study. We believe that its feedback inhibition may affect the synthesis of 2-phenylethyl alcohol. We use this Mutant Feedback-Resistant Version of aroG for to resisting feedback inhibition and improving enzyme activity. This is the composite of our Improvement of Standard Biological Parts. Our team successfully constructed a pathway for the synthesis of 2-phenylethanol in Escherichia coli. When cultured for 60 hours, the maximum concentration of 2-phenylethanol was 67.16mg/L.After 60 hours,the concentration of 2-phenylethanol decreases because of toxicity of 2-PE accumulated in E. coli and metabolism.
The standard curve for determination of 2-phenylethanol yield
The experimental 2-phenylethanol yield
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 450
Illegal EcoRI site found at 665
Illegal XbaI site found at 87
Illegal XbaI site found at 941 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 450
Illegal EcoRI site found at 665 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 450
Illegal EcoRI site found at 665
Illegal BamHI site found at 738 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 450
Illegal EcoRI site found at 665
Illegal XbaI site found at 87
Illegal XbaI site found at 941 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 450
Illegal EcoRI site found at 665
Illegal XbaI site found at 87
Illegal XbaI site found at 941 - 1000COMPATIBLE WITH RFC[1000]
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