Device

Part:BBa_K176221

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-10-08)
Revision as of 11:35, 8 October 2009 by Registry (Talk | contribs)

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cell density->GFP: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFP

GFP = (Cell Density * pCon 0.01) + Input AHL

High level GFP inhibits cell growth.

This part is both a cell density reporter and a cell density controller.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 856
    Illegal NheI site found at 879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 698
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1834
    Illegal BsaI.rc site found at 2578


[edit]
Categories
Parameters
None