DNA
yjbB

Part:BBa_K4252001

Designed by: Penghui Li   Group: iGEM22_HNU_China   (2022-09-25)
Revision as of 10:17, 11 October 2022 by Lipenghui (Talk | contribs)


phosphate exportation

As a phosphorus export element.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 426
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1174


Introduction

So far, only one type of P exporting protein, YjbB, has been reported in E. coli.The mechanism of phosphorus export by yjbB is still unclear. But based on experimental phenomena we choose YjbB as the phosphorus output element in our project.

Engineering

DNA fragments containing yjbB was amplified from E. coli MG1655 genomic DNA using the primers yjbB-5’/yjbB-3’.The PCR fragments were inserted into the Xbal/Xhol sites of pET-22b(+).

Result

Results of pET-22b(+)-yjbB sequencing (primers: YjbB-2-5′, YjbB-2-3′)


Figure 1: results-of-pet-22b-yjbB-sequencing.
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Categories
Parameters
None