Coding

Part:BBa_K4244044

Designed by: Max Allewijn   Group: iGEM22_Wageningen_UR   (2022-10-08)
Revision as of 10:08, 11 October 2022 by ArthurVancolen (Talk | contribs)

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Split UM (b1-9:b10-11)

This split Ultramarine was split between the 9th and 10th beta-sheet. It was created as control for flipUM (BBa_K4244046). It expresses Ultramarine in two fragments which can come together again to form functional UM.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 423
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 753
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 423
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 753
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 423
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 423
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 753
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 423
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 753
    Illegal AgeI site found at 543
    Illegal AgeI site found at 884
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None