Part:BBa_K4244021

cold shock protein A promoter

5'UTR of the E. coli cold shock protein cspA gene (1). The PcspA is a regulatory region that has a high rate of transcription but contains a long 5′ UTR of 159 bp that, at 37°C, acquires an unstable secondary structure and leads to its degradation. On the other hand, at lower temperatures, it forms a stable configuration, which allows translation (2).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1.Mitta, M., Fang, L., & Inouye, M. (1997). Deletion analysis of cspA of Escherichia coli: requirement of the AT-rich UP element for cspA transcription and the downstream box in the coding region for its cold shock induction. Molecular microbiology, 26(2), 321–335. https://doi.org/10.1046/j.1365-2958.1997.5771943.x 2.Stirling, F., Bitzan, L., O'Keefe, S., Redfield, E., Oliver, J., Way, J., & Silver, P. A. (2017). Rational Design of Evolutionarily Stable Microbial Kill Switches. Molecular cell, 68(4), 686–697.e3. https://doi.org/10.1016/j.molcel.2017.10.033