Part:BBa_K4165175

Biobrick His - Tau

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), Tau (BBa_K4165009), and T7 terminator (BBa_K731721).

Source

Synthesized

Usage and Biology

In the AD brain, tau Hyperphosphorylation is considered the main cause of AD progression, it may alter the protein's shape and charge, which in turn causes the microtubule-binding domain to become exposed and allow tau to self-assemble and form oligomers characterized to be a neurofibrillary tangle. According to several studies, the polymerized tau (neurofibrillary tangles) is inert since it does not bind to tubulin or encourage its assembly into microtubules, the His Tag for the purification of the protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 169
  Illegal AgeI site found at 355
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 333
  Illegal BsaI site found at 1245
  Illegal BsaI.rc site found at 45
  Illegal SapI.rc site found at 519
  

Dry Lab

Modeling

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab.

                 Figure 1. this figure shows the results from the transcription and translation code showing 
                the variation of mRNA and protein concentrations with time compared with the wet lab results.

WetLab Results

                                    Figure 2. Transformed plate of His Tau + pGS-21a