Device
Part:BBa_K176200
Designed by: Danqian Liu, Zongxiao He, Hao Jiang Group: iGEM09_USTC (2009-10-08)
Cell density controller: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)
Cell Death = (Cell Density * pCon 0.70) + Input AHL
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 856
Illegal NheI site found at 879 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 698
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2323
Illegal BsaI.rc site found at 1834
[edit]
Categories
Parameters
None |