Regulatory

Part:BBa_K4207000

Designed by: Jesper Mickos   Group: iGEM22_Aboa   (2022-09-26)
Revision as of 09:15, 11 October 2022 by Krisse (Talk | contribs)


T7max promoter

This promoter is an optimized version of the standard T7 promoter for usage in cell-free transcription systems.


Usage and Biology

This promoter recruits the standard T7 RNA-polymerase, so it can be used in standard applications, which take advantage of the T7 transcription reactions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

5. References

Deich, C., Cash, B., Sato, W., Sharon, J., Aufdembrink, L., Gaut, N., Heili, J., Stokes, K., Engelhart, A., Adamala, K. (2021) T7Max transcription system. https://doi.org/10.1101/2021.10.17.464727


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Parameters
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