Primer

Part:BBa_K4361112

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-08)
Revision as of 08:41, 11 October 2022 by Larsvdb (Talk | contribs)


Blc operator SDM primer F

A site-directed mutagenesis primer, used to induce the deletion of adenine 20 in the Blc operator sequence (Part:BBa_K4361111) during PCR, in conjunction with the reverse primer Part:BBa_K4361113. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence (Part:BBa_K4361114) may act as a negative control when testing the binding properties of BlcR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None