Plasmid

Part:BBa_K4361106

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-07)
Revision as of 08:29, 11 October 2022 by Larsvdb (Talk | contribs)


pET-11a with BlcR with 6xHis-tag and TEV protease cleavage site

This composite part shows our expression system for E. coli codon optimized BlcR with purification tag and cleavage site (Part:BBa_K4361103) in the pET-11a expression plasmid (Part:BBa_K4361105), for use in PURE reactions. We chose this system as it allowed us to express BlcR and test its properties in a single system (see also Part:BBa_K4361115 and Part:BBa_K4361117).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
    Illegal NheI site found at 5639
    Illegal NheI site found at 6446
    Illegal PstI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 5528
    Illegal BamHI site found at 5672
    Illegal BamHI site found at 6581
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
    Illegal NgoMIV site found at 3394
    Illegal NgoMIV site found at 3748
    Illegal NgoMIV site found at 3908
    Illegal NgoMIV site found at 5496
    Illegal NgoMIV site found at 5830
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1242
    Illegal SapI.rc site found at 2324
    Illegal SapI.rc site found at 6341


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