Composite

Part:BBa_K4147012

Designed by: Denisse Chacon Ramirez   Group: iGEM22_Tec-Chihuahua   (2022-10-09)
Revision as of 08:20, 11 October 2022 by Denisse (Talk | contribs)


Up part of cassette for siRNA production of RXLR gene for P. capsici

Upper part of the cassette for siRNA expression. This part is part of the construct expressing the siRNA flanking the mRNA of the RXLR gene of P. capsici. It is cmpossed of a reverse T7 terminator with a RBS (BBa_K4147015) and a T7 promotor and RBS (BBa_K525998).

Usage and Biology

To produce the double-stranded siRNAs, an expression system has been designed and must be assembled. The assembly method is based on the one shown by the iGEM Ecuador 2021 team.

The figure below shows the assembly strategy that is planned to be used.

action.
Assembly strategy for siRNA production

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None