Coding

Part:BBa_K4164997:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-10-02)
Revision as of 07:46, 11 October 2022 by JieYang (Talk | contribs)


RXR-3*GS Linker-ddRFPB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1867
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 357
    Illegal AgeI site found at 1384
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 739


Design Notes

The stop codon of the RXR was deleted and a flexible linker (3*GGGGS) was added between RXR and ddRFPB1.


Source

This sequence is composed of BBa_K528004

https://parts.igem.org/wiki/index.php?title=Part:BBa_K528004 https://parts.igem.org/Part:BBa_K4164013

References